Obstetrics and Gynecology

doi: 10.25005/2074-0581-2020-22-1-22-27
IMMUNOHISTOCHEMICAL MARKERS AND HISTOLOGICAL AND MORPHOLOGICAL CHANGES IN THE PLACENTA OF WOMEN, WHO GAVE BIRTH TO CHILDREN WITH ANENCEPHALY

V.Yu. Melnikova, M.F. Dodkhoeva

Department of Obstetrics and Gynecology № 1, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikistan

Objective: To study immunohystochemical markers and features of histological and morphological changes in the placenta of women who have given birth to children with anencephaly.

Methods: 15 women with anencephaly in the fetus (the main group) and 20 women, who gave birth to practically healthy children (control group), were examined. All women were tested for tumor marker alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG), morphological examination of placenta and levels of apoptosis, definition expression Nkx-2.2 in vascular endothelial cells of the villous chorion of the placenta of women in norm, and with congenital malformation (CM) of the central nervous system (CNS), in particular – anencephaly in the fetus.

Results: A feature of the histochemical characteristics of placenta in women who have given birth to children with anencephaly is the predominance of chronic compensated deficiency with manifestations of dyscirculatory disorders. Statistically significant reduction of Nkx-2.2 expression levels in the vascular endothelial cells of the villous chorion placenta of women who gave birth to children with anencephaly, confirms the role of this factor in the differentiation of nervous structures. An inverse correlation between the level of Nkx-2.2 expression in the placenta and the level of AFP in the blood serum of pregnant women at the beginning of the second trimester of pregnancy determines the level of AFP as the most significant marker of the development of anencephaly in the fetus.

Conclusion: Based on the conducted studies it is possible to assume the participation of the placenta in the formation of abnormalities of the CNS of fetuses and newborns. Consequently, properly organized antenatal surveillance with mandatory definition of AFP level at 14 weeks of pregnancy will improve the quality of care for women at risk development of CM of the CNS of the fetus, in particular – anencephaly

Keywords: Immunohistochemical markers, diagnostics of anencephaly, expression of Nkx-2.2 in the placenta, degree of apoptosis in the placenta.

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References
  1. Melnikova VYu, Dodkhoeva MF, Kolobov AV. Nekotorye aspekty vrozhdoynnykh porokov razvitiya tsentral’noy nervnoy sistemy u ploda [Some aspects of congenital malformations of the central nervous system in the fetus]. Doklady Akademii nauk Respubliki Tadzhikistan. 2015;58(8):751-6.
  2. Soprunova IV, Belopasov VV. Rasprostranyonnost’ vrozhdyonnykh porokov tsentral’noy nervnoy sistemy na territorii Astrakhanskoy oblasti [The prevalence of congenital malformations of the central nervous system in the territory of the Astrakhan region]. Astrakhanskiy meditsinskiy zhurnal. 2011;6(3):197-202.
  3. Price EM, Peсaherrera MS, Portales-Casamar E, Pavlidis P, Macfadden DE. Profiling placental and fetal DNA methylation in human neural tube defects. Epigenetics & Chromatin. 2016;9(6):1-14. Available from: https://doi. org/10.1186/s13072-016-0054-8.
  4. Smirnova AYu, Antonenko FF, Khamoshina MB, Dudar OA. Prenatal’naya diagnostika u beremennykh yunogo vozrasta v Primorskom krae [Prenatal diagnosis in young pregnant women in the Primorsky Territory]. Byulleten’ VSNTs SO RAMN. 2006;1:94-6.
  5. Novikova IV, Venchikova NA, Solovyeva IV, Lishtvan LM. Morfologicheskiy spektr patologii pri ul’trazvukovom vyyavlenii porokov tsentral’noy nervnoy sistemy v I trimestre beremennosti [Morphological spectrum of pathology with ultrasound detection of defects of the central nervous system in the first trimester of pregnancy]. Prenatal’naya diagnostika. 2015;14(3):205-14.
  6. Gavrilova TV. Vozmozhnosti ul’trazvukovogo issledovaniya v diagnostike vrozhdyonnykh porokov razvitiya ploda [The possibilities of ultrasound in the diagnosis of congenital malformations of the fetus]. Byulleten’ meditsinskikh Internet konferentsiy. 2013;3(3):661.
  7. Umarbaeva DA. Patomorfologicheskie izmeneniya posleda pri vrozhdyonnykh porokakh razvitiya ploda [Pathomorphological changes in the placenta with congenital malformations of the fetus]. Vestnik Kyrgyzsko-Rossiyskogo slavyanskogo universiteta. 2014;14(5):185-7.
  8. Trubnikova LI. Diagnosticheskaya znachimost’ kompleksnogo issledovaniya vrozhdyonnykh porokov razvitiya tsentral’noy nervnoy sistemy ploda [The diagnostic significance of a comprehensive study of congenital malformations of the central nervous system of the fetus]. Ul’yanovskiy mediko-biologicheskiy zhurnal. 2012;3:55-60.
  9. Shabaldin AV. Rol’ alfa-fetoproteina v patogeneze vrozhdyonnykh porokov razvitiya ploda [The role of alpha-fetoprotein in the pathogenesis of congenital malformations of the fetus]. Mat’ i ditya v Kuzbasse. 2007;3:16-9.
  10. Hall JW, Denne N, Minardi JJ, Williams D, Balcik BJ. Check the head: emergency ultrasound diagnosis of fetal anencephaly. Journal of Emergency Medicine. 2016;17(4):460-3.
  11. Kolobov AV, Pinchukova AZ, Karev EV. Morfologicheskie osobennosti platsenty cheloveka pri kariotipirovannoy trisomii 21-y kromosomy [Morphological features of human placenta with karyotyped trisomy of the 21st chromosome]. Klinicheskaya patofiziologiya. 2018;2:42-5.
  12. Demikova NS, Kobrinskiy BA, Lapina AS. Chastota i struktura vrozhdyonnykh porokov razvitiya po dannym monitoringa [The frequency and structure of congenital malformations according to monitoring.]. Meditsinskaya genetika. 2009;4(5):178.
  13. Sherstnyova OV. Prenatal’naya ul’trazvukovaya diagnostika vrozhdyonnykh porokov razvitiya tsentral’noy nervnoy sistemy [Prenatal ultrasound diagnosis of congenital malformations of the central nervous system]. Meditsinskiy sovet. 2012;1:31-7.
  14. Nizyaeva NV, Sukhachyova TV, Kulikova GV, Nagovitsyna MN, Kan NE, Bayev OR, i dr. Morfologicheskie osobennosti mezenkhimal’nykh kletok stromy vorsin khoriona [Morphological features of mesenchymal cells of the stroma of the chorionic villi]. Vestnik Rossiyskoy akademii meditsinskikh nauk. 2017;72(1):76-83.
  15. Shevchenko AA. Vrozhdyonnye poroki razvitiya tsentral’noy nervnoy sistemy: kliniko-nevrologicheskie aspekty i problemy prenatal’noy diagnostiki [Congenital malformations of the central nervous system: clinical and neurological aspects and problems of prenatal diagnosis]. Mezhdunarodnye obzory: klinicheskaya praktika i zdorov’e. 2014;2:34-50.
  16. Al-Obaidly S, Thomas J, Abu Jubara M, Al Ibrahim A, Al-Belushi M, Saleh N, et al. Anencephaly and obstetric outcome beyond the age of viability. Journal of Perinatal Medicine. 2018;46(8):885-888. Available from: https://doi. org/10.1515/jpm-2017-0363.
  17. Bardi F, Smith E, Kuilman M. Early detection of structural anomalies in a primary care setting in the Netherlands. Fetal Diagnosis and Therapy. 2019;46(1):12-9. Available from: https://doi.org/10.1159/000490723.
  18. Flood TJ, Rienks ChM, Flores AL, May KT, Frochnert BK, Rutkowski RE, et al. Using state and provincial surveillance programs to reduce risk of recurrence of neural tube defects in the United States and Canada: A missed opportunity? Birth Defects Res A Clin Mol Teratol. 2016;106(11):875-80. Available from: https://doi.org/10.1002/bdra.23576.
  19. Holz A, Kollmus H, Ryge J, Niederkofler V, Diaz J, Ericson J, et al. The transcription factors Nkx2.2 and Nkx2.9 play a novel role in floor plate development and commissural axon guidance. Development. 2010;24:4249-60. Available from: https://doi.org/10.1242/dev.053819: 10.1242/dev.053819.

Authors' information:


Melnikova Veronika Yuryevna
Assistant of the Department of Obstetrics and Gynecology № 1, Avicenna Tajik State Medical University
ORCID ID: 0000-0002-9514-5100
Author ID: 1029649
SPIN: 5917-8251
E-mail: veronika.melnikova.86@mail.ru

Dodkhoeva Munavvara Fayzulloevna
Academician of the Academy of Medical Sciences of the Republic of Tajikistan, Doctor of Medical Sciences, Full Professor, Professor of the Department of Obstetrics and Gynecology № 1, Avicenna Tajik State Medical University
ORCID ID: 0000-0001-9373-4318
Author ID: 313026
SPIN: 9749-6174
E-mail: dodkho2008@mail.ru

Information about support in the form of grants, equipment, medications

The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:


Melnikova Veronika Yuryevna
Assistant of the Department of Obstetrics and Gynecology № 1, Avicenna Tajik State Medical University

734003, Republic of Tajikistan, Dushanbe, Rudaki Ave., 139

Tel.: +992 (935) 180220

E-mail: veronika.melnikova.86@mail.ru

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