Dentistry

doi: 10.25005/2074-0581-2022-24-1-59-65
Use a cu stom-mad e CAD probe holders for periodontal microcircu lation assessment by laser Doppler flowmetry

A. A. Flenkin, S.N. Ermolyev

Department of Periodontology, Moscow State University of Medicine and Dentistry named after A. I. Evdokimov, Moscow, Russian Federation

Objective: To perform laser Doppler flowmetry (LDF) periodontal microcirculation assessment using custom-made, computer-aided-designed probe holders manufactured by stereolithography (SLA).

Methods: 66 young people aged 21-23 years old with clinically healthy periodontium were examined. The first group consisted of subjects where a custom-made probe holder was fabricated using a silicone impression mould technique. The second group consisted of subjects where a custom-made computer-aided-design probe holder made of a photopolymer by SLA was used. The basic microcirculation parameters were analysed to evaluate periodontal microcirculation by the LDF method: the PM – average perfusion value in periodontal tissues; δ – the average square deviation of the amplitude of blood flow fluctuations from the arithmetic mean value, Kv – coefficient of variation (%). Statistical processing of the results was carried out using the software package Statistica 13.0 (StatSoft Inc, USA).

Results: Statistical analysis results indicated that the functional characteristics of the periodontal microcirculation using various custom-made fibre optic probe holders used in capillary blood flow monitoring had significant differences p<0.05. The value of the median PM when using a silicone holder in group I was 1.6 times lower than when using a photopolymer holder in group II. The median value of the σ index when using a silicone holder in group I was 2.3 times higher than when using a photopolymer holder in group II. The median value of the Kv when using a silicone holder in group I was 2.5 times higher than when using a photopolymer holder in group II.

Conclusion: A photopolymer probe holder, unlike a silicone one, provides a constant fixed distance between the periodontal tissues and the LDF probe, prevents movement or probe displacement, and makes it possible to avoid pressure on the gingival tissue, ensuring high accuracy of laser diagnostics

Keywords: Functional diagnostics, LDF, microcirculation, periodontium, CAD, stereolithography.

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References
  1. Orekhova LYu, Loboda ES, Yamanidze NA, Beryozkina IV. Znachenie mikrotsirkulyatsii v diagnostike i dinamike lecheniya vospalitel'nykh zabolevaniy parodonta [The importance of microcirculation in the diagnosis and dynamics of treatment of inflammatory periodontal diseases]. Meditsina: teoriya i praktika. 2019;4(5):404-5.
  2. Gorbatova EA, Mikhaylova AB, Kozlova MV, Yakushechkina EP. Osobennosti gemodinamiki mikrotsirkulyatornogo rusla desny patsientov s vospalitel'nymi zabolevaniyami parodonta na etapakh kompleksnogo lecheniya s primeneniem sovremennykh fitopreparatov [Features of hemodynamics of the microcirculatory bed of the gums of patients with inflammatory periodontal diseases at the stages of complex treatment with the use of modern phytopreparations]. Kremlyovskaya meditsina. Klinicheskiy vestnik. 2018;1:159-64.
  3. Kovalevskiy AM, Pototskaya AV, Podberyozkina LA, Borisova EG, Sharafutdinova DO. Vozmozhnosti primeneniya fizicheskikh metodov v kompleksnom lechenii vospalitel'nykh zabolevaniy parodontA. Chast' 3 [The possibilities of using physical methods in the complex treatment of inflammatory periodontal diseases. Part 3]. Institut stomatologii. 2019;2:90-3.
  4. Stepanov EA, Kurashvili LV, Mikulyak NI, Moiseev YaP, Kinzirskiy AS. Osobennosti mikrotsirkulyatsii v parodonte pri razlichnykh sistemnykh zabolevaniyakh [Features of microcirculation in periodontal disease in various systemic diseases]. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Meditsinskie nauki. 2021;2:137-50. Available from: https://doi.org/10.21685/2072-3032- 2021-2-13
  5. Yanushevich OO, Dmitrieva LA. Parodontologiya. Natsional'nye rukovodstva [Periodontology. National guidelines]. Moscow, RF: GEOTAR-Media; 2018. 752 p.
  6. Erganova OI, Kornev NA, Ermolyev SN, Volkov EA. Metody funktsional'noy diagnostiki v stomatologii sravnitel'naya otsenka regionarnogo krovotoka v tkanyakh parodonta i pul'py zuba [Methods of functional diagnostics in dentistry comparative assessment of regional blood flow in periodontal tissues and tooth pulp]. Stomatologiya dlya vsekh. 2018;4:41-3.
  7. Tarasenko SV, Krechina EK, Zagorskiy SV. Sostoyanie mikrogemodinamiki i oksigenatsii v slizistoy obolochke al'veolyarnogo grebnya posle konturnoy plastiki desny pri dental'noy implantatsii [The state of microhemodynamics and oxygenation in the mucous membrane of the alveolar ridge after contouring of the gum during dental implantation]. Stomatologiya. 2020;99(5):46-9. Available from: https://doi.org/10.17116/stomat20209905146
  8. Krupatkin AI, Sidorov VV. Funktsional'naya diagnostika sostoyaniya mikrotsirkulyatornotkanevykh sistem. Kolebaniya, informatsiya, nelineynost'. Rukovodstvo dlya vrachey [Functional diagnostics of the state of microcirculatory and tissue systems. Fluctuations, information, non-linearity. Guide for doctors]. Moscow, RF: Lenand; 2013. 496 p.
  9. Ermolyev SN, Zholudev SE, Eroshkina EA, Delets AV. Sposob pozitsionirovaniya svetovodnogo zonda pri ispol'zovanii dopplerovskoy floumetrii v stomatologii [Method of positioning a light guide probe when using Doppler flowmetry in dentistry]. Patent Rossiyskoy Federatsii № 2400133. 27.09.2010.
  10. Flenkin AA, Ermolyev SN, Yanushevich OO, Vinnichenko YuA, Merkusheva NA, Boyarskiy YuV, i dr. Sposob pozitsionirovaniya optovolokonnogo zonda dlya pretsizionnoy opticheskoy diagnostiki gemodinamiki i kislorodnogo rezhima tkaney parodonta na osnove CAD tekhnologii [Method for positioning a fiberoptic probe for precision optical diagnostics of hemodynamics and oxygen recovery of periodontal tissues based on CAD technology]. Patent Rossiyskoy Federatsii № 2758660. 01.11.2021.
  11. Ivanova VA, Borisov VV, Platonova VV, Danshina SD. Vysokaya tochnost' konstruktsii pri primenenii 3D pechati v implantologii [High accuracy of designs when using 3D printing in implantology]. Aktual'nye problemy meditsiny. 2020;43(1):93-101. Available from: https://doi.org/10.18413/2687-0940- 2020-43-1-93-101
  12. Bavrina A P. V pomoshch' issledovatelyu sovremennye pravila ispol'zovaniya metodov opisatel'noy statistiki v medikobiologicheskikh issledovaniyakh [To help the researcher, modern rules for the use of descriptive statistics methods in biomedical research]. Meditsinskiy al'manakh. 2020;2:95-104.
  13. Petri A, Sabin K. Naglyadnaya meditsinskaya statistika. Uchebnoe posobie [Clear medical statistics. Textbook]. Moscow, RF: GEOTAR-Media; 2021. 221 p.
  14. Michel MC, Murphy TJ, Motulsky H J. New author guidelines for displaying data and reporting data analysis and statistical methods in experimental biology. Mol Pharmacol. 2020;7(1):49-60. Available from: https://doi.org/10.1124/ mol.119.118927.9
  15. Buttarazzi D, Pandolfo G, Porzio GC. A boxplot for circular data. Biometrics. 2018;74(4):1492-501. Available from: https://doi.org/10.1111/biom.12889
  16. Moore JPR, Dyson A, Singer M, Fraser J. Microcirculatory dysfunction and resuscitation: Why, when, and how. British Journal of Anaesthesia. 2015;115(3):366- 75.
  17. Jacob M, Chappell D, Becker BF. Regulation of blood flow and volume exchange across the microcirculation. Critical Care. 2016;20:13.
  18. Schmidt Harald HHW, Feelisch M. Red blood cell-derived nitric oxide bioactivity and hypoxic vasodilation. Circulation. 2019;139(23):2664-7.

Authors' information:


Flenkin Andrey Andreevich
PhD Applicant, Department of Periodontology, Moscow State University of Medicine and Dentistry named after A. I. Evdokimov
ORCID ID: 0000-0001-6023-1677
SPIN: 5598-6092
Author ID: 1071516
E-mail: Dr.Flenkin@gmail.com

Ermolyev Sergey Nikolaevich
Doctor of Medical Sciences, Professor of the Department of Periodontology, Moscow State University of Medicine and Dentistry named after A. I. Evdokimov
ORCID ID: 0000-0002-4219-3547
SPIN: 7109-4050
Author ID: 636370
E-mail: esn54@mail.ru

Information about support in the form of grants, equipment, medications

The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:


Flenkin Andrey Andreevich
PhD Applicant, Department of Periodontology, Moscow State University of Medicine and Dentistry named after A. I. Evdokimov, Moscow

127473, Russian Federation, Moscow, Delegatskaya str., 20/1

Тel.: +7 (925) 9810590

E-mail: Dr.Flenkin@gmail.com

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