Internal Medicine

doi: 10.25005/2074-0581-2019-21-1-38-42
FEATURES OF FREE RADICAL OXIDATION AND METABOLIC ACTIVITY OF VASCULAR ENDOTHELIUM IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

A.M. Saburova1, Kh.R. Nasyrdzhonova1, Kh.Yo. Sharipova2, M.B. Kurbanova1

1Department of Biochemistry, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikistan
2Department of Propaedeutics of Internal Medicine, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikistan

Objective: To study the features of free radical oxidation and metabolic activity of the vascular endothelium in patients with chronic obstructive pulmonary disease (COPD).

Methods: The results of the examination of 58 patients with COPD aged 28-54 years who were treated at the City Medical Center № 2 named after Academician K.T. Tadzhiev of Dushanbe city. There were 32 men (52.2%), and 26 women (44.8%). Free radical oxidation and the antioxidant system were predetermined by the content of reactive oxygen species (ROS), the amount of malondialdehyde (MDA), and the activity of superoxide dismutase (SOD). The state of the vascular endothelium was assessed by the content of C-reactive protein (CRP), fibrinogen, von Willebrand factor, total cholesterol, LDLP, and HDLP.

Results: In patients with COPD in the serum, the intensification of free radical oxidation was noted in the form of an increase in the content of ROS by 30.1%, MDA by 125% and a decrease in SOD activity by 43.2% compared to the control. As a result, there is an imbalance between oxidants and antioxidants with the development of oxidative stress, which plays an important role in the pathogenesis of COPD. A study of the state of the vascular endothelium in patients with COPD revealed an increase in CRP by 16 times, fibrinogen by 61.5%, von Willebrand factor by 33.7%, total cholesterol by 30%, LDLP by 61.5% against a decrease in HDLP by 43.4% compared with control data. A correlation of ROS with fibrinogen (r=0.733817) was noted, which ultimately leads to endothelial dysfunction and the development of thromboembolic complications

Conclusion: The course of COPD is accompanied by the development of a systemic inflammatory response, the starter of the mechanism which is oxidative stress, accompanied by an intensification of free radical oxidation, depletion of the antioxidant system. Oxidative stress contributes to damage to the vascular endothelium, the criteria of which are increased levels of CRP, fibrinogen, von Willebrand factor and an atherogenic lipid spectrum disorder (increased LDLP and total cholesterol, along with a decrease in HDLP)

Keywords: COPD, free radical oxidation, endothelium, endothelial dysfunction, biochemical studies.

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References
  1. Novikova NE, Kudryasheva IA, Akhmineeva AKh. Okislitel’nyy stress pri KHOBL [Oxidative stress in COPD]. Astrakhanskiy meditsinskiy zhurnal. 2012;7(3):87-90.
  2. Chuchalin AG. Khronicheskaya obstruktivnaya bolezn’ lyogkikh i soputstvuyushchie zabolevaniya [Chronic obstructive pulmonary disease and associated diseases]. Pul’monologiya. 2008;2:5-14
  3. Menshikova EB, Zenkov NK, Lankin VZ, Bondar IA, Trufakin VA. Okislitel’nyy stress: Patologicheskie sostoyaniya i zabolevaniya [Oxidative stress: Pathological conditions and diseases]. Novosibirsk, RF: AR-TA; 2008. 284 p.
  4. Ovcharenko SI, Kapustina VI. Okislitel’nyy stress i antioksidantnaya terapiya bol’nykh khronicheskoy obstruktivnoy bolezn’yu lyogkikh [Oxidative stress and antioxidant therapy in patients with chronic obstructive pulmonary disease]. Bolezni organov dykhaniya. 2011;1:7-11.
  5. Makarova MA, Avdeev SN, Chuchalin AG. Arterial’naya rigidnost’ i endotelial’naya disfunktsiya u patsientov s khronicheskoy obstruktivnoy bolezn’yu lyogkikh [Arterial stiffness and endothelial dysfunction in patients with chronic obstructive pulmonary disease]. Pul’monologiya. 2011;6:78-84.
  6. Balabolkin II. Bronkhial’naya astma [Bronchial asthma]. Moscow, RF; MIA: 2015. 141 p.
  7. Artykova TK, Ismoilov KI. Perekisnoe okislenie lipidov i antioksidantnaya zashchita organizma detey s vegeto-sosudistoy distoniey [Lipid peroxidation and antioxidant protection of children with vegetative-vascular dystonia]. Vestnik Avitsenny [Avicenna Bulletin]. 2015;3;117-20.
  8. Ismoilov KI, Saburova AM, Sharipova MM. Antioksidantnaya zashchita i osobennosti perekisnogo okisleniya lipidov pri bronkhial’noy astme u detey [Antioxidant protection and features of lipid peroxidation in children with bronchial asthma]. Vestnik Avitsenny [Avicenna Bulletin]. 2017;19(1):73-7. Available from: http://dx.doi.org/10.25005/2074-0581-2017-19-1-73-77.
  9. Soodaeva SK, Nikitina LYu, Klimanov IA. Mekhanizmy razvitiya oksidativnogo stressa pod vozdeystviem aeropollyutantov okruzhayushchey sredy: potentsial sredstv antioksidantnoy zashchity [Mechanisms of development of oxidative stress under the influence of environmental polluting agents: the potential of antioxidantprotection agents]. Pul’monologiya. 2015;6:736-42.
  10. Shapovalova TG, Ryabova AYu, Plastinina ES, Ponamaryova OA, Shelobanova NV, Shashina MM, i dr. Khronicheskaya obstruktivnaya bolezn’ lyogkikh u patsientov kardiologicheskogo profilya: problemy diagnostiki i lecheniya [Chronic obstructive pulmonary disease in cardiac patients: problems of diagnosis and treatment]. Byulleten’ sovremennoy klinicheskoy meditsiny. 2016;9(1):79-83.
  11. Phaniendra A. Free radical’s properties, sources, targets, the implication in various diseases. Indian Clin Buochem. 2015;30(1):11-26.
  12. Nadeen A. Airway and systemic oxidant-antioxidant dysregulation in asthma a possible scenario of oxidant spill over from lung into blood. Pulm Pharmacol Ther. 2014;29(1):31-40.
  13. Wilson D, Badri M, Maartens G. Performance of serum C-reactive protein as a screening test for smear-negative tuberculosis in an ambulatory high HIV prevalence population. PLoS ONE. 2011;6:15248.
  14. Poplavskaya EE, Ris MI. Sostoyanie funktsii endoteliya i aktivnosti fagotsitoza pri khronicheskoy obstruktivnoy bolezni lyogkikh i ishemicheskoy bolezni serdtsa [State of endothelial function and phagocytosis activity in chronic obstructive pulmonary disease and coronary heart disease]. Zhurnal Grodnenskogo gosudarstvennogo meditsinskogo universiteta. 2010;1:29-31
  15. Avdeev SN, Makarova MA. Arterial’naya rigidnost’ i endotelial’naya disfunktsiya u patsientov s khronicheskoy obstruktivnoy bolezn’yu lyogkikh [Arterial stiffness and endothelial dysfunction in patients with chronic obstructive pulmonary disease]. Pul’monologiya. 2011;4:109-17.
  16. Pushkaryova TA, Koryakina LB, Runovich AA, Kurilskaya TE, Pivovarov YuI. Kriterii otsenki disfunktsii endoteliya i puti eyo korrektsii [Criteria for assessing endothelial dysfunction and ways to correct it]. Klinicheskaya laboratornaya diagnostika. 2008;5:3-7.
  17. Pribylov SA, Ovsyannikov AG, Prusakova OYu, Aliullin RV, Shabanov EA, Semidotskaya IYu. Rol’ lyogochnoy gipertenzii, endotelial’noy disfunktsii, mediatorov vospaleniya v patogeneze khronicheskoy obstruktivnoy bolezni lyogkikh, assotsiirovannoy s ishemicheskoy bolezn’yu serdtsa [The role of pulmonary hypertension, endothelial dysfunction, inflammatory mediators in the pathogenesis of chronic obstructive pulmonary disease associated with ischemic heart disease]. Chelovek i ego zdorov’e. 2011;4:151-4.

Author information:


Saburova Anna Mukhammadievna
Doctor of Biological Sciences, Full Professor, Professor of the Department of Biochemistry, Avicenna Tajik State Medical University

Nasyrdzhonova Khursand Rakhimovna
Senior Lecturer, Department of Biochemistry, Avicenna Tajik State Medical University

Sharipova Khursand Yodgorovna
Doctor of Medical Sciences, Full Professor, Professor of the Department of Propedeutics of Internal Diseases, Avicenna Tajik State Medical University

Kurbanova Masuda Bakievna
Candidate of Biological Sciences, Associate Professor, Department of Biochemistry, Avicenna Tajik State Medical University

Information about support in the form of grants, equipment, medications

The authors did not receive financial support from manufacturers of medicines and medical equipment.

Conflicts of interest: No conflict

Address for correspondence:


Saburova Anna Mukhammadievna
Doctor of Biological Sciences, Full Professor, Professor of the Department of Biochemistry, Avicenna Tajik State Medical University

734003, Republic of Tajikistan, Dushanbe, Rudaki Ave., 139

Tel.: +992 (917) 241175

E-mail: n_hursand@mail.ru

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