EXPERIMENTAL RESEARCH

Pathophysiology

doi: 10.25005/2074-0581-2023-25-1-84-93
IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES)

M.A. Olimov1, M.U. Sharofova2,3, F.M. Khodzhaeva4, A.D. Kholbekov1, J.T. Bobokalonov5

1Department of Morphology, Faculty of Medicine, Tajik National University, Dushanbe, Republic of Tajikistan
2Institute of Avicenna's Medicine and Pharmacology, Dushanbe, Republic of Tajikistan
3Center for Research of Innovative Technologies of the National Academy of Sciences of Tajikistan, Dushanbe, Republic of Tajikistan
4Department of Pharmacology with a Course of Clinical Pharmacology, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikistan
5Laboratory of Biopolymers, Institute of Chemistry named after V.I. Nikitin, National Academy of Sciences of Tajikistan, Dushanbe, Republic of Tajikistan

Objective: To study the morphophysiological features of burn wound healing in rats using a polysaccharide gel form based on pectin and polysaccharide from apricot gum with encapsulated sea buckthorn oil.

Methods: The study was carried out on two groups of laboratory rats with 18 animals in each group. A skin burn injury was inflicted in both groups (for 5 seconds at a temperature of 150°C). In the main group, in order to facilitate regeneration, a new gel form based on a polysaccharide composite with encapsulated sea buckthorn oil was applied to the burn site. In the control group, instead of the gel, a 3% hydrogen peroxide solution was applied to the surface of the wound. The skin, subcutaneous tissue, and muscles were sampled for morphological studies, sample size being 2×2 cm. The tissue was sampled within unchanged skin and processed for histological examination. Histological slides were stained with hematoxylin-eosin and viewed under Axiostar PLUS microscope (Carl Zeiss, Germany).

Results: In the control group, physiological wound healing took place which corresponds to incomplete healing (reparative regeneration) and the formation of scars. In the main group after the gel treatment, the formation of granulation tissue within the epidermis, dermis, and subcutaneous fat was accelerated. It was noted that the use of the proposed gel limited destructive changes, and contributed to the steady maturation of young connective tissue, ensuring the process of re-epithelialization and complete closure of the defect

Conclusion: The proposed polysaccharide gel with encapsulated sea buckthorn oil has great potential for use as a dressing for burn wounds to expedite their healing.

Keywords: Burn wound, wound healing, polysaccharide gel, pectin, apricot gum, sea buckthorn oil (Hippophae rhamnoides).

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Author information:


Olimov Makhmadrakhim Amonalievich,
Applicant, Department of Morphology, Faculty of Medicine, Tajik National University
ORCID ID: 0000-0001-6045-3333
SPIN-код: 1922-4850
Author ID: 627641
E-mail: mahmarahimolimzoda@gmail.com

Sharofova Mizhgona Umedzhonovna,
Doctor of Medical Sciences, Director of Institute of Avicenna's Medicine and Pharmacology; Principal Researcher, Center for Research of Innovative Technologies of the National Academy of Sciences of Tajikistan
ORCID ID: 0000-0002-7155-7194
SPIN-код: 1803-2448
Author ID: 1019198
E-mail: mijgona72@mail.ru

Kholbekov Asliddin Dzhumaevich,
Candidate of Biological Sciences, Head of the Department of Morphology, Faculty of Medicine, Tajik National University
ORCID ID: 0000-0003-0365-8396
E-mail: farang71@mail.ru

Bobokalonov Jamshed Tolehmurodovich,
Candidate of Biological Sciences, Leading Researcher, Laboratory of Biopolymers, Institute of Chemistry named after V.I. Nikitin, National Academy of Sciences of Tajikistan
ORCID ID: 0000-0002-0829-9364
SPIN-код: 6733-6345
Author ID: 1149144
E-mail: jamshedbt@gmail.com

Information about support in the form of grants, equipment, medications

Experiments with animals were carried out with the financial support of the International Scientific and Technical Center within the framework of the T-1419 project. The study presented in this publication was also supported by the Fogarty International Center of the National Institutes of Health under project D43TW009672. The content is the sole responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Conflicts of interest: No conflict

Address for correspondence:


Sharofova Mizhgona Umedzhonovna
Doctor of Medical Sciences, Director of Institute of Avicenna's Medicine and Pharmacology; Principal Researcher, Center for Research of Innovative Technologies of the National Academy of Sciences of Tajikistan

734063, Republic of Tajikistan, Dushanbe, Ayni str., 299/3

Tel.: +992 (918) 614123

E-mail: mijgona72@mail.ru

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