REVIEW ARTICLE

General Surgery

doi: 10.25005/2074-0581-2025-27-4-1000-1010
CONTEMPORARY DIAGNOSIS OF HEPATIC HYDATID ECHINOCOCCOSIS

D.V. PAKHNOV1, G.D. ODISHELASHVILI1, D.L. LUTSKY2, L.R. PAKHNOVA3

1Department of Surgical Diseases, Faculty of Dentistry, Astrakhan State Medical University, Astrakhan, Russian Federation
2Department of Biological Chemistry and Clinical Laboratory Diagnostics, Astrakhan State Medical University, Astrakhan, Russian Federation
3Department of Radiation Diagnostics, Astrakhan State Medical University, Astrakhan, Russian Federation

This literature review summarizes the latest diagnostic methods for diagnosing hepatic cystic echinococcosis (HCE). The study involved a systematic search of bibliographic databases, including eLibrary, PubMed, and Medline, covering the period from January 2004 to December 2024. The keywords used in the search included "диагностика эхинококкоза", "cytokines", "laboratory diagnostics", "hydatid cyst", "инструментальные методы диагностики", "instrumental diagnostic methods", and "viability of hydatid".

A detailed examination of the diagnosis and treatment of echinococcosis reveals several unresolved critical issues. One major challenge is the development of laboratory methods to assess the viability of echinococcal cysts (ECs), which is crucial for establishing the indications for chemotherapy. Prioritizing the development of new diagnostic methods and the improvement of existing ones for this disease is essential. Research focused on laboratory and imaging techniques to differentiate between active and inactive ECs in humans can significantly inform treatment strategies for patients. Thus, the need for a more in-depth analysis of the unresolved issues in the diagnosis and treatment of echinococcosis is evident.

Keywords: Echinococcosis, epidemiology, laboratory diagnostics, instrumental diagnostics..

Download file:


References
  1. Wen H, Vuitton L, Tuxun T, Li, Vuitton DA, Zhang W, et al. Echinococcosis: Advances in the 21st century. Clin Microbiol Rev. 2019;32(2):e00075-18. https:// doi.org/10.1128/CMR.00075-18
  2. Shabunin AV, Lebedev SS, Kovalenko YuA, Karpov AA. Sovremennoe sostoyanie problemy khirurgicheskogo lecheniya ekhinokokkoza pecheni [Current state of the problem of surgical treatment of liver echinococcosis]. Annaly khirurgicheskoy gepatologii. 2021;26(4):87-96. https://doi.org/10.16931/19955464.2021-4-87-96
  3. Arzhigitova AK, Shapekova NL. Monitoring epidemiologicheskoy situatsii ekhinkokkoza po Zapadno-Kazahstanskoy oblasti [Monitoring the epidemiological situation of echinococcosis in the West Kazakhstan region]. Vestnik Severno-Kazakhstanskogo universiteta im. M. Kozybaeva. 2021;1:79-83.
  4. Pakhnov DV, Odishelashvili GD, Odishelashvili LG, Serdyukov VG. Kombinirovannyy podkhod k lecheniyu gidatidnogo ekhinokokkoza pecheni [Combined approach to treatment of hydatid echinococcosis of the liver]. Astrakhanskiy meditsinskiy zhurnal. 2017;2(4):13-20.
  5. Pakhnov DV, Kuchin YuV, Odishelashvili GD. Sposob likvidatsii bol'shikh polostey posle ekhinokokkektomii pecheni [Method for eliminating large cavities after liver echinococcectomy]. Astrakhanskiy meditsinskiy zhurnal. 2014;3(9):118-22.
  6. Sapaev DSh, Ruzibaev RYu, Yakubov FR. Sovremennaya kompleksnaya diagnostika i khirurgicheskoe lechenie oslozhnyonnykh form ekhinokokkoza pecheni [Modern comprehensive diagnostics and surgical treatment of complicated forms of liver echinococcosis]. Vestnik khirurgii im. Grekova. 2018;177(4):19-22. https://doi.org/10.24884/0042-4625-2018-177-4-19-22
  7. Dragomeretskaya AG, Trotsenko OE, Bebenina LA, Ignatieva ME, Kurganova OP, ZaytsevaTA, i dr. Seroepidemiologicheskiy monitoring za tsistnym ekhinokokkozom v Dal'nevostochnom federal'nom okruge [Seroepidemiological monitoring of cystic echinococcosis in the Far Eastern Federal District]. Dal’nevostochnyy meditsinskiy zhurnal. 2018;3:33-9.
  8. Odishelashvili GD, Pakhnov DV, Kostenko NV, Nozdrin VM, Odishelashvili LG. Redkoe oslozhnenie ehinokokkoza [A rare complication of echinococcosis]. Khirurgiya. Zhurnal im. N.I. Pirogova. 2023;3:72-5. https://doi.org/10.17116/ hirurgia202303173
  9. Pakhnov DV, Odishelashvili GD, Odishelashvili LG. Gidatidnyy ekinokokkoz v ambulatornoy praktike vracha-khirurga [Hydatid cists in the outpatient practice of a surgeon]. Ambulatornaya khirurgiya. 2020;3-4:135-9. https://doi. org/10.21518/1995-1477-2020-3-4-135-139
  10. Pagnozzi D, Tamarozzi F, Roggio АM, Tedde V, Addis MF, Pisanu S, et al. Structural and immunodiagnostic characterization of synthetic antigen B subunits from Echinococcus granulosus and their evaluation as target antigens for cyst viability assessment. Clin Infect Dis. 2018;66(9):1342-51. https://doi.org/10.1093/cid/ cix1006
  11. Manterola C, Vial M, Melo A, Oberg C, Fonseca F. Viability and fertility of human hepatic hydatid cysts. World J Surg. 2006;30(2):227-32. https://doi.org/10.1007/ s00268-005-0215-6
  12. Azizzoda ZA, Kurbonov KM, Ruziboyzoda KR, Rasulov NA, Ali-Zade SG. Znachenie laboratornykh pokazateley v diagnostike oslozhnyonnogo ehinokokkoza pecheni [Importance of laboratory parameters in the diagnostics of complicated liver echinococcosis]. Vestnik Avitsenny [Avicenna Bulletin]. 2019;21(4):655-60. https://doi.org/10.25005/2074-0581-2019-21-4-655-660
  13. Arakelyan RS, Shendo GL, Maslyaninova AE, Kovalenko AV, Kiselyova AA, Arakelyants OA. Serologicheskie metody issledovanija v diagnostike parazitarnykh bolezney [Serological research methods in the diagnosis of parasitic diseases]. Mezhdunarodnyy naucno-issledovatel'skiy zhurnal. 2021;110 (8-2):78-82. https://doi.org/10.23670/IRJ.2021.110.8.051
  14. Akimniyazova BB, Kausova GK, Eshmuratov TSh, Eleusizov AM, Enin EA. O trudnostyakh diagnostiki sochetannogo oslozhnyonnogo mnozhestvennogo ekhinokokkoza lyogkik [On the difficulties of diagnosis of combined complicated multiple pulmonary echinococcosis]. Meditsina (Almaty). 2019;202(4):69-74. https://doi.org/10.31082/1728-452X-2019-202-4-69-73
  15. Siles-Lucas M, Casulli A, Conraths FJ, Müller N. Laboratory diagnosis of Echinococcus spp. in human patients and infected animals. Adv Parasitol. 2017;96:159-257. https://doi.org/10.1016/bs.apar.2016.09.003
  16. Rialch A, Tigga MN, Arun A, Samanta S, Raina OK. Evaluation of diagnostic potential of Echinococcus granulosus recombinant EgAgB8/1, EgAgB8/2 and EPC1 antigens for cystic echinococcosis in goats. J Pure Appl Microbiol. 2017;11(1):441-8. https://doi.org/10.22207/JPAM.11.1.58
  17. Rialch A, Raina OK, Tigga MN, Anandanarayanan A, Ganaie ZA, Aftab A, et al. Evaluation of Echinococcus granulosus recombinant EgAgB8/1, EgAgB8/2 and EPC1 antigens in the diagnosis of cystic echinococcosis in buffaloes. Vet Parasitol. 2018;252:29-34. https://doi.org10.1016/j.vetpar.2018.01.020
  18. Faramarzi T, Mobasheri M, Yoosefy A, Valadkhani Z. Expression and purification of truncated recombinant B8/1 protein of Echinococcus granulosus for diagnosis of hydatid infection in human. Acta Tropica. 2019;191:139-45. https://doi. org/10.1016/j.actatropica.2018.12.039
  19. Lissandrin R, Tamarozzi F, Piccoli L, Tinelli C, De Silvestri A, Mariconti M. Factors Influencing the serological response in hepatic Echinococcus granulosus infection. Am J Trop Med Hyg. 2016;94(1):166-71. https://doi.org/10.4269/ajtmh.15-0219
  20. Retamozo AR, Agüero FA, Fuchs AG, Maglioco A. Serological diagnosis in hydatidosis: Searching standardized antigenic support. Systematic review and meta-analysis. Interam J Health Sci. 2021;1:89-108. https://doi.org/10.59471/ ijhsc202141
  21. Chzhao AV, Ikramov RZ. Khirurgicheskoe lechenie ekhinokokkoza pecheni [Surgical treatment of liver echinococcosis]. Consilium Medicum. Surgery. 2016; 2:15-7.
  22. Kasyev NB, Aytnazarov MS, Nurbekova AN. Immunnokhemilyuminesstentsiya v diagnostike ekhinokokkoza [Immune chemiluminescence in the diagnosis of echinococcosis]. Vestnik nauki i obrazovaniya. 2016;19(7):96-9.
  23. da Silva ED, Cancela M, Monteiro KM, Ferreira HB, Zaha A. Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways. PLoS Negl Trop Dis. 2018;12(5):e0006473. https://doi.org/10.1371/journal. pntd.0006473
  24. Silva-Alvarez V, Folle AM, Ramos AL, Kitano ES, Iwai LK, Corraliza I, et al. Echinococcus granulosus antigen B binds to monocytes and macrophages modulating cell response to inflammation. Parasit Vectors. 2016;9:69. https:// doi.org/10.1186/s13071-016-1350-7
  25. Chun-Seob A, Huixia C, Jeong-Geun K, Xiumin H, Xiao M, Young-An B, et al. An Echinococcus multilocularis antigen B3 proteoform that shows specific antibody responses to active-stage alveolar echinococcosis. J Clin Microbiol. 2015;53(10):3310-7. https://doi.org/10.1128/JCM.01362-15
  26. Sadjjadi FS, Mohammadzadeh T, Jafari SH, Sharifi Y, Deilami HN, Hafezi F, et al. Comparison of native hydatid cyst fluid (HCF), lyophilized HCF, antigen B (AgB) and lyophilized AgB (LAgB) originated from Echinococcus granulosus sensu stricto for sero-diagnosis of active, transitional and inactive human liver cystic echinococcosis. Iran J Public Health. 2023;52(8):1764-72. https://doi. org/10.18502/ijph.v52i8.13416
  27. Calu V, Enciu O, Toma EA, Parvuleţu R, Piriianu DC, Miron A. Complicated liver cystic echinococcosis – A comprehensive literature review and a tale of two extreme cases. Tomography. 2024;10(6):922-34. https://doi.org/10.3390/ tomography10060070
  28. Grimm J, Beck A, Nell J, Schmidberger J, Hillenbrand A, Beer AJ, et al. Combining computed tomography and histology leads to an evolutionary concept of hepatic alveolar echinococcosis. Pathogens. 2020:4;9(8):634. https://doi.org/10.3390/ pathogens9080634
  29. Stojkovic’ M, Gottstein B, Weber TF, Junghanss T. Cystic, alveolar and neotropical echinococcosis. Manson's Tropical Diseases. 2024:797-821. https://doi. org/10.1016/B978-0-7020-7959-7.00061-0
  30. Ma G, Holland CV, Wang T, Hofmann A, Fan C, Maizels RM, et al. Human toxocariasis. The Lancet Infectious Diseases. 2018;18(1):14-24. https://doi. org/10.1016/S1473-3099(17)30331-6
  31. Grishina EA. Antigeny i metabolity gel'mintov kak reguliruyushchie faktory protivoparazitarnogo immuniteta [Antigens and metabolites of helminths as regulating factors of antiparasitic immunity]. Epidemiologiya i infektsionnye zabolevaniya. Aktual’nye voprosy. 2016;2:58-63.
  32. Zhi-Dan L, Xiao-Jin M,Shuai Y, Dong W, Bin X, Jian G, et al. Multiplex cytokine and antibody profile in cystic echinococcosis patients during a three-year followup in reference to the cyst stages. Parasit Vectors. 2020;13(1):133. https://doi. org/10.1186/s13071-020-4003-9
  33. Shi C, Zhou X, Yang W, Wu J, Bai M, Zhang Y, et al. Proteomic analysis of plasmaderived extracellular vesicles from mice with Echinococcus granulosus at different infection stages and their immunomodulatory functions. Front Cell Infect Microbiol. 2022;12:805010. https://doi.org/10.3389/fcimb.2022.805010
  34. Saftawy EA, Abdelraouf A, Elsalam MA, Zakareya P, Fouad A, Albadawi EA. Autoimmunity in human CE: Correlative with the fertility status of the CE cyst. Helminthologia. 2022;59(1):1-17. https://doi.org/10.2478/helm-2022-0011
  35. Biranvand E, Rafiei A, Beiromvand M, Amari A, Bahraini A, Motamedfar A. Cytokine profiles in peripheral blood mononuclear cells from patients with cystic echinococcosis. Comp Immunol Microbiol Infect Dis. 2020;70:101469. https:// doi.org/10.1016/j.cimid.2020.101469
  36. Kurzantseva OM. Ekhinokokkoz pecheni: vozmozhnosti instrumental'nykh metodov diagnostiki, slozhnosti v differentsial'noy diagnostike, klinicheskiy sluchay [Echinococcosis of the liver: Possibilities of instrumental diagnostic methods, difficulties in differential diagnosis, clinical case]. SonoAce Ultrasound. 2021;33:19-38.
  37. Basarukin MA, Petrova EB, Tyurina NV. Klinicheskiy sluchay gidatidnogo ekhinokokkoza pecheni v praktike vracha ul'trazvukovoy diagnostiki [Clinical case of liver hydatid echinococcosis from the experience of the ultrasonic diagnostics practitioner]. Radiologiya-praktika. 2021;85(1):92-6.
  38. Hoy X, Shi Y, Kang X, Rousu Z, Li D, Wang M, et al. Echinococcus granulosus: The establishment of the metacestode in the liver is associated with control of the CD4+ T-cell-mediated immune response in patients with cystic echinococcosis and a mouse model. Front Cell Infect Microbiol. 2022;12:983119. https://doi. org/10.3389/fcimb.2022.983119
  39. Zhang YY, Ma QF, Chen XX, Ying CH, Ma YL, Li P, et al. Value of contrastenhanced ultrasonography and acoustic radiation force impulse elastography in identification of boundary range and viability of hepatic alveolar echinococcosis. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2019;32(1):91-3. https://doi. org/10.16250/j.32.1374.2018087
  40. Galyavin AV. Gidatidoznyy ekhinokokkoz pecheni i lyogkik: diagnosticheskie i terapevticheskie aspekty [Hydatid Echinococcusis of the liver and lungs: Diagnostic and therapeutic aspects]. Infektsionnye bolezni: novosti, mneniya, obuchenie. 2024;13(3):101-9. https://doi.org/10.33029/2305-3496-2024-13-3101-109
  41. Stepanova YuA, Goncharov AB, Chzhao AV. Ul'trazvukovaya diagnostika na etapakh lecheniya ekhinokokkoza pecheni [Ultrasound diagnostics at the stages of treatment of liver echinococcosis]. Annaly khirurgicheskoy gepatologii. 2022;15(3):244-53. https://doi.org/10.18499/2070-478X-2022-15-3-244-253
  42. Egorkina AB, Stepanova YuA, Karmazanovskiy GG, Kalinin DV, Chzhao AV. Rasprostranyonnyy rak lyogkogo v sochetanii s ekhinokokkozom pecheni [Disseminated lung cancer in combination with liver echinococcosis]. Meditsinskaya vizualizatsiya. 2021:25(2):125-32. https://doi.org/10.24835/16070763-941
  43. Dmitrashchenko AA, Alekhnovich AV, Esipov AA, Algoritm luchevoy diagnostiki pri ekhinokokkoze razlichnoy lokalizatsii [Algorithm of radiodiagnostics for echinococcosis of the different localization]. Gospital'naya meditsina: nauka i praktika. 2019;1(4):5-10
  44. Karmazanovskiy GG, Stepanova YuA, Kondratiev EV, Stashkiv VI. Ekhinokokkoz pecheni: trudnosti diagnostiki na rannikh stadiyah razvitiya i pri oslozhnyonnom techenii (obzor literatury) [Hepatic echinococcosis: Difficulties in diagnosis at the early stages of progression and with complications (literature review)]. Annaly khirurgicheskoy gepatologii. 2021;26(4):18-23. https://doi.org/10.16931/19955464.2021-4-18-23
  45. Abdurakhmanov DSh, Khaydarova LO. Evoluutsiya metodov diagnostiki i khirurgicheskogo lecheniya ekhinokokkoza pecheni [Evolution of methods of diagnosis and surgical treatment of liver echinococcosis]. Dostizheniya nauki i obrazovaniya. 2020;70(16):70-6.
  46. Polskiy VS, Soldatchenkov AS. Preimushchestva i nedostatki nekotorykh metodov issledovaniya organizma [Advantages and disadvantages of some methods of studying the body]. Integrativnye tendentsii v meditsine i obrazovanii. 2021;3:6773.
  47. Caraiani C, Petresc B, Dong Y, Dietrich CF. Contraindications and adverse effects in abdominal imaging. Med Ultrason. 2019;21(4):456-63. http://doi.org/10.11152/ mu-2145
  48. Busko EA, Kozubova KV, Bagnenko SS, Karachun AM, Burovik IA, Goncharova AB, i dr. Sravnitel'nyy analiz effektivnosti KT i kontrastno usilennogo UZI v diagnostike metastazov kolorektal'nogo raka v pecheni [Comparative assessment of diagnostic value of computed tomography and contrast-enhanced ultrasound in colorectal cancer liver metastases diagnosis]. Annaly khirurgicheskoy gepatologii.2022;27(1):22-32. https://doi.org/10.16931/1995-5464.2021-1-22-32

Authors' information:


Pakhnov Dmitry Vladimirovich,
Candidate of Medical Sciences, Associate Professor of the Department of Surgical Diseases, Faculty of Dentistry, Astrakhan State Medical University
ORCID ID: 0000-0002-7803-8661
SPIN: 8269-5808
Author ID: 772584
E-mail: Pahnov1@mail.ru

Odishelashvili Givi Domentievich,
Doctor of Medical Sciences, Full Professor, Head of the Department of Surgical Diseases, Faculty of Dentistry, Astrakhan State Medical University
Scopus ID: 57214529428
ORCID ID: 0000-0002-4303-3764
SPIN: 9769-9368
Author ID: 674864
E-mail: Givi64@mail.ru

Lutsky Dmitry Leonidovich,
Doctor of Medical Sciences, Associate Professor, Professor of the Department of Biological Chemistry and Clinical Laboratory Diagnostics, Astrakhan State Medical University
Scopus ID: 6603221790
ORCID ID: 0000-0002-1412-3322
SPIN: 8826-1441
Author ID: 159920
E-mail: dmitry.lutsky@bk.ru

Pakhnova Liya Ruslanovna,
Candidate of Medical Sciences, Associate Professor of the Department of Radiation Diagnostics, Astrakhan State Medical University
Scopus ID: 57209748537
ORCID ID: 0000-0002-4021-325Х
SPIN: 3631-1791 Author ID: 1003362 E-mail: Pahnova@mail.ru

Information about support in the form of grants, equipment, medications

The research was carried out in accordance with the research plan of Astrakhan State Medical University (state registration number – 405.01). The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:


Pakhnov Dmitry Vladimirovich
Candidate of Medical Sciences, Associate Professor of the Department of Surgical Diseases, Faculty of Dentistry, Astrakhan State Medical University

414000, Russian Federation, Astrakhan, Bakinskaya str., 121

Tel.: +7 (927) 6602782

E-mail: Pahnov1@mail.ru


This work is licensed under a Creative Commons Attribution 4.0 International License.

Materials on the topic: