ORIGINAL RESEARCH
General Surgery
doi: 10.25005/2074-0581-2026-28-3-720-727
CLINICAL EFFICACY OF PRP THERAPY AND CHANGES IN GROWTH FACTOR LEVELS IN PATIENTS WITH CHRONIC NON-HEALING WOUNDS

A.O. OKHUNOV, SH.A. BOBOKULOVA

Tashkent State Medical University, Tashkent, Republic of Uzbekistan

Objective: To evaluate the clinical efficacy of platelet-rich plasma (PRP) therapy and its effects on serum concentrations of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), and transforming growth factor beta (TGF-β) in patients with chronic non-healing wounds of various etiologies.

Methods: This prospective randomized study included 60 patients with chronic non-healing wounds of various etiologies. The main group (MG) comprised 30 patients who received PRP therapy, while the control group (CG) included 30 patients who received standard treatment. Treatment efficacy was assessed based on changes in wound area, the rate of epithelialization, and serum concentrations of the growth factors VEGF, PDGF, and TGF-β. Biomarker concentrations were measured at admission and on day 14 using enzyme-linked immunosorbent assay (ELISA).

Results: In the MG, wound area decreased significantly by day 7 and further decreased by day 14 (p<0.001). The CG also showed a tendency toward reduced wound area (p=0.045); however, after Bonferroni correction (p<0.017), this decrease was not statistically significant. At the between-group comparison on day 14, wound area was significantly smaller in the TG than in the CG (p=0.008). In the TG, PRP therapy was associated with statistically significant increases in VEGF (p<0.001), PDGF (p=0.004), and TGF-β (p=0.039) levels by day 14 compared with baseline values. The CG showed no statistically significant changes (p>0.05). At the between-group comparison on day 14, serum concentrations of VEGF, PDGF, and TGF-β were significantly higher in the TG than in the CG (p<0.001, p=0.002, and p=0.037, respectively).

Conclusion: PRP therapy significantly improves clinical and molecular indicators of tissue regeneration and accelerates the healing of chronic non-healing wounds. These findings support the potential for broader use of PRP therapy in the clinical management of patients with chronic non-healing wounds.

Keywords: Chronic non-healing wounds, platelet-rich plasma, growth factors, tissue regeneration, molecular mechanisms.

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Authors' information:

Okhunov Alisher Oripovich,
Doctor of Medical Sciences, Full Professor, Head of the Department of General and Pediatric Surgery, Tashkent State Medical University
Researcher ID: HKO-2361-2023
Scopus ID: 6508358215
ORCID ID: 0000-0003-3622-6805
SPIN: 2943-1604
Author ID: 989103
E-mail: general-surgery@mail.ru

Bobokulova Shokhista Abdualimovna,
PhD, Senior Lecturer of the Department of General and Pediatric Surgery, Tashkent State Medical University
Scopus ID: 57234567890
ORCID ID: 0000-0002-9521-1411
SPIN: 7940-1870
Author ID:
E-mail: shokhista.bobokulova@tma.uz

Information about support in the form of grants, equipment, medications

The research was carried out in accordance with the research plan of Tashkent State Medical University (state registration number – 142001/20018). The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:

Okhunov Alisher Oripovich
Doctor of Medical Sciences, Full Professor, Head of the Department of General and Pediatric Surgery, Tashkent State Medical University

100109, Republic of Uzbekistan, Tashkent, Farobi str., 2

Tel.: +998 (909) 474568

E-mail: general-surgery@mail.ru


This work is licensed under a Creative Commons Attribution 4.0 International License.

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