EXPERIMENTAL RESEARCH
Phthisiatry
doi: 10.25005/2074-0581-2025-27-4-922-928
DETERMINATION OF THE ACTIVE COMPOUNDS IN THE EXTRACT OF THE BERBERIS VULGARIS LEAVES PREPARATION FOR ITS APPLICATION TO PREVENT A PEPTIC GASTRIC ULCER

M. KAPITONOVA1, R.N. ALYAUTDIN2,3, S.L. MOROKHINA4, E.V. SERGUNOVA2, I.B. BROHI1, S.B. TALIP1, N. RAMLI1, A.A.I. IBRAHIM5

1Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kota Samarahan, Malaysia
2I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation
3Scientific Center for Expert Evaluation of Medicinal Products, Moscow, Russian Federation
4N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
5Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia

Objective: To establish a reliable method of preparing Berberis vulgaris leaves extract and determining its active components.

Methods: The spectrophotometric method was used to determine the active components in the alcohol extract of Berberis vulgaris leaves extract.

Results: Chemical analysis showed that the Berberis vulgaris leaf extract contains 0.48±0.09% of flavonoids, 0.07±0,02% of alkaloids, and 8.05±0.17% of tanning substances, which make it suitable for application as prevention of gastric ulcer development.

Conclusion: This study established an efficient, sensitive, and selective method for simultaneous determination of the flavonoids, alkaloids, and tanning substances in the Berberis vulgaris leaves extract, which could be successfully applied to prevent gastric ulcer development in preclinical trials.

Keywords: Berberis vulgaris, leaf extract, spectrophotometry, gastric ulcer.

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Authors' information:

Kapitonova Marina,
MD, PhD, Professor, Professor of Anatomy, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak
Researcher ID: Y-6429-2018
Scopus ID: 8854275100
ORCID ID: 0000-0001-6055-3123
E-mail: kmarina@unimas.my

Alyautdin Renad Nikolaevich,
MD, PhD, Professor of the Pharmacology Department, I.M. Sechenov First Moscow State Medical University; Head, Scientific Center for Expert Evaluation of Medicinal Products
Researcher ID: L-9261-2014
Scopus ID: 6701792451
ORCID ID: 0000-0002-4647-977X
E-mail: alyautdin@mail.ru

Morokhina Svetlana Lvovna,
PhD, Methodologist, Faculty of Training of Staff of the Highest Qualification, N.I. Pirogov Russian National Research Medical University
Researcher ID: P-6813-2018
ORCID ID: 0000-0003-1065-9610
E-mail: morokhinas@mail.ru

Sergunova Ekaterina Vyacheslavovna,
PhD, Professor of the Department of Pharmaceutical Natural Studies, I.M. Sechenov First Moscow State Medical University
ORCID ID: 0000-0002-7194-5525
E-mail: sergunova_e_v@staff.sechenov.ru Brohi Imam Bux,
MD, Associate Professor of the Family Medicine, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak
ORCID ID: 0000-0001-7574-2154
E-mail: bimam@unimas.my

Talip Saiful Bahri,
MD, MSc, Senior Lecturer, Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak
Researcher ID: 57191838596
Scopus ID: 57191838596
ORCID ID: 0000-0001-9470-4559
E-mail: tsbahri@unimas.my

Ramli Norhida,
MD, MSc, Lecturer of Anatomy, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak
Researcher ID 56893285800
Scopus ID: 56893285800
ORCID ID: 0000-0002-8764-5340
E-mail: rnorhida@unimas.my

Ibrahim Abdel Aziz Ibrahim,
MD, Associate Professor, Pharmacology and Toxicology Department, Faculty of Medicine, Umm Al-Qura University
Scopus ID: 57190843246
ORCID ID: 0000-0001-5757-8653
E-mail: iamustafa@uqu.edu.sa

Information about support in the form of grants, equipment, medications

The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:

Talip Saiful Bahri,
MD, MSc Senior Lecturer, Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak

UNIMAS campus, Kota Samarahan, 94300, Sarawak, Malaysia

Tel.: +60 (198) 279003

E-mail: tsbahri@unimas.my


This work is licensed under a Creative Commons Attribution 4.0 International License.

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