Pharmacology

doi: 10.25005/2074-0581-2019-21-3-450-454
ANTI-TUMOR ACTIVITY OF DRY EXTRACT PADUS GRAYANAE MAXIM IN VITRO

I.Z. Ismailov, A.Z. Zurdinov, T.S. Sabirova

Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academy, Bishkek, Kyrgyz Republic

Objective: To study the antitumor activity of dry extract Padus Grayanae Maxim in vitro conditions.

Methods: The object of the study was dry extract Padus Grayanae Maxim, obtained by lyophilic drying method from water-alcohol extraction of plant medicinal raw materials and standardized in content chlorogenic and coffee acids. The study of the cytotoxicity of Padus Grayanae Maxim dry extract conducted on cultures of tumor cells that were derived from the American Type Culture Collection (ATCC). Cell mortality was assessed using the MTT test to form a non-soluble formazan.

Results: It has been established that the studied phytoextraction an antitumor effect in relation to the lines of tumor cells of human cervical adenocarcinoma (HeLa), stomach adenocarcinoma (AGS), human pancreatic carcinoma (MiaPaCa2) and rhabdomyosarcoma of mice (RD). At the same time, fairly high specificity of the action of the studied phytoextraction revealed in relation to the tumor cells adenocarcinoma of the human stomach (AGS). The cell line of tumor cells of human hepatocellular carcinoma (HepG2) has shown resistance to dry extract Padus Grayanae Maxim.

Conclusions: The antitumor effect of the dry extract Padus Grayanae Maxim in vitro conditions has been revealed. At the same time, a specific action against various tumor strains may be due to flavonoids, chlorogenic and coffee acids, which have a wide range of cytotoxicity.

Keywords: Dry extract Padus Grayanae Maxim, antitumor activity, flavonoids, chlorogenic acid, coffee acid.

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Authors informations:

Ismailov Isabek Zaylidinovich Doctor of Pharmaceutical Sciences, Associate Professor, Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academy ORCID ID: 0000-0002-1468-8251 Author ID: 877010 SPIN: 9540-1750 E-mail: ism-isa@mail.ru

Zurdinov Ashirali Zurdinovich Doctor of Medical Sciences, Professor of the Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academy ORCID ID: 0000-0002-7110-0901 Author ID: 877038 SPIN: 3719-0291 E-mail: a-zurdinov@yandex.ru

Sabirova Tamara Semyonovna Candidate of Medical Sciences, Associate Professor, Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academy ORCID ID: 0000-0002-3709-0244 Author ID: 877003 SPIN: 1684-5129 E-mail: sabirova_ts@mail.ru

Address for correspondence:

Ismailov Isabek Zaylidinovich
Doctor of Pharmaceutical Sciences, Associate Professor, Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academ

720020, Kyrgyz Republic, Bishkek, Akhunbaev Street, 92

Tel.: +996 (559) 639 777

E-mail: ism-isa@mail.ru

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