Pharmacology
doi: 10.25005/2074-0581-2024-26-1-67-75
IN VITRO EVALUATION OF ANTITHROMBOTIC AND ANTICOAGULANT ACTIVITY OF HERBAL SOURCES
1Department of Biochemistry, Bashkir State Medical University, Ufa, Russian Federation
2Department of Pharmacognosy with the Course of Botany and Basics of Phytotherapy, Bashkir State Medical University, Ufa, Russian Federation
3Department of Organization of Pharmaceutical Business and Pharmacognosy, Osh State University, Osh, Republic of Kyrgyzstan
4Department of Pharmacology with the Course of Clinical Pharmacology, Bashkir State Medical University, Ufa, Russian Federation
5Department of Physiology and General Biology of the Institute of Nature and Human, Ufa University of Science and Technology, Ufa, Russian Federation
6Educational and Experimental Farm of Ufa Forestry Technical College, Ufa, Russian Federation
Objective: In vitro evaluate the antiaggregation and anticoagulation properties of aqueous plant extracts, including Viburnum opulus flowers and fruits, Urtica dioica leaves, Coffea arabica leaves, and shepherd's purse (Capsella bursa-pastoris) herb.
Methods: In vitro assessment of anticoagulation activity was conducted by examining the impact on activated partial thromboplastin time (APTT), prothrombin time (PT), and fibrinogen levels. Anti-aggregation activity was evaluated using the Born turbidimetric method.
Results: The research found that the phytoextracts examined did not impact the concentration of fibrinogen and PT. However, they did slightly prolong the APTT, and this effect was statistically significant compared to the control. Coffea arabica leaves, and Viburnum opulus fruit extracts showed substantial anticoagulant activity (30% and 29% sodium heparin, respectively; p-value <0.05 for both). Extracts of Viburnum opulus fruits and Coffea arabica leaves significantly prolong the latent period before collagen-induced platelet aggregation by up to 116.7% and 118.8% of the intact platelet index, respectively. Every sample analyzed showed a significant decrease in the maximum platelet aggregation amplitude compared to the control. The most notable impact was with Urtica dioica and Coffea arabica leaf extracts, which reduced the percentage to 85.9% and 89.2% of the control, respectively. These extracts exhibited 77.6% and 59.7% of the effectiveness of acetylsalicylic acid, with Coffea arabica extract showing a significant difference (p<0.05) compared to the reference drug. All plant extracts, except Capsella bursa-pastoris extract, which reduced this indicator, significantly prolonged the time needed to reach maximum aggregation. Coffea arabica extract had the most significant impact, showing 223.8% of the efficacy of acetylsalicylic acid (p<0.05). The extracts (excluding Capsella bursa-pastoris) significantly decreased the platelet aggregation rate. The most notable impact was observed with extracts of Viburnum opulus flowers and Coffea arabica leaves, which yielded an inhibitory effect on the rate of aggregation of acetylsalicylic acid (-10%/min relative to intact platelets, p<0.05), reducing aggregation rate by -5.2% and -6%/min compared to control, respectively.
Conclusion: The samples analyzed in our study show weak anticoagulation activity, with the most noticeable effects in extracts of coffee leaves and viburnum fruits. Additionally, we found that the antiaggregation activity of the extracts of coffee leaves, nettles, and viburnum fruits was much more substantial, comparable to or even surpassing the effect of acetylsalicylic acid in some cases.
Keywords: Anticoagulation properties, antiaggregation activity, Viburnum opulus, Urtica dioica, Coffea arabica, Capsella bursa-pastoris.
References
- Matos MJ. Coumarin and its derivatives – Editorial. Molecules. 2021;26(20):6320. https://doi.org/10.3390/molecules26206320
- Tanashyan MM, Raskurazhev AA, Kuznetsova PI. Aspirin: legenda prodolzhaetsya [Aspirin: The legend continues]. Profilakticheskaya meditsina. 2018;21(5):124-9. https://doi.org/10.17116/profmed201821051124
- Pereira B, Brazón J, Rincón M, Vonasek E. Browplasminin, a condensed tannin with antiplasmin activity isolated from an aqueous extract of Brownea grandiceps Jacq. flowers. J Ethnopharmacology. 2017;198:182-290. https:// doi.jrg/10.1016/j.jep.2017.01.012
- Zhang Y, Xie P, Guo X, Kang W. Procoagulant substance and mechanism of Myristica fragrans. J Med Food. 2016;19(11):1065-73. https://doi.org/10.1089/ jmf.2016.3700
- Fan PC, Ma HP, Hao Y, He XR, Sun AJ, Jiang W, et al. A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata. J Ethnopharmacology. 2016;187:232-8. https://doi. org/10.1016/j.jep.2016.04.016
- Pawlaczyka I, Capek P, Czerchawsk L, Bijak J, Lewik Tsirigotisa M, Pliszczak-Krold A, et al. An anticoagulant effect and chemical characterization of Lythrum salicaria L. glycoconjugates. Carbohydr Polym. 2011;86(1):277-84. https://doi. org/10.1016/j.carbpol.2011.04.048
- Wang T, Guo R, Zhou G, Zhou X, Kou Z, Sui F, et al. Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) F.H. Chen: A review. J Ethnopharmacology. 2016;188:234-58. https://doi. org/10.1016/j.jep.2016.05.005
- Muschin T, Budragchaa D, Kanamoto T, Nakashima H, Ichiyama K, Yamamoto N, et al. Chemically sulfated natural galactomannans with specific antiviral and anticoagulant activities. Int J Biol Macrmol. 2016;89:410-20. https://doi. org/10.1016/j.ijomac.2016.05.005
- Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod. 2016;79(3):629-61. https://doi.org/10.1021/acs.jnatprod.5b01055
- Diamond BJ, Bailey MR. Gingko biloba: Indications, mechanisms, and safety. Psychiatr Clin North Am. 2013;36(1):73-83. https://doi.org/10.1016/j. psc.2012.12.006
- Kajszczak D, Zaklos-Szyda M, Podsedek A. Viburnum opulus l. – a review of phytochemistry and biological effects. Nutrients. 2020;12(11):3308. https://doi. org/10.3390/nu12113398
- Polka D, Podsedek A, Koziolkiewicz M. Comparison of chemical composition and antioxidant capacity of fruit, flower and bark of Viburnum opulus. Plant Foods for Human Nutrition. 2019;74(3):436-42. https://doi.org/10.1007/ s11130-019-00759-1
- Kraujalyte V, Venskutonis PR, Pukalskas A, Cesoniene L, Daubaras R. Antioxidant properties and polyphenolic compositions of fruits from different European cranberrybush (Viburnum opulus L.) genotypes. Food Chem. 2013;141(4):3695-702. https://doi.org/10.1016/j.foodchem.2013.06.054
- Ersoy N, Ercisli S, Gundogdu M. Evaluation of European cranberry bush (Viburnum opulus L.) genotypes for agro-morphological, biochemical and bioactive characteristics in Turkey. Folia Hort. 2017;29:181-8. https://doi.org/10.1515/ fhort-2017-0017
- Perova IB, Zhogova AA, Cherkashin AV, Eller KI, Ramenskaya GV. Biologicheski aktivnye veshchestva plodov kaliny obyknovennoy [Biologically active substances of Viburnum opulus fruits]. Khimiko-farmatsevticheskiy zhurnal. 2014;48(5):32-9. https://doi.org/10.30906/0023-1134-2014-48-5-32-39
- Al-Snafi AE. The chemical constituents and pharmacological effects of Capsella bursa-pastoris – A review. International Journal of Pharmacology and Toxicology. 2015;5(2):76-81.
- Cha J, Kim DH, Lee T, Subedi L, Kim S, Lee K. Phytochemical constituents of Capsella bursa-pastoris and their anti-inflammatory activity. Natural Product Sciences. 2018;24(2):132. https://doi.org/10.20307/nps.2018.24.2.132
- Patay EB, Bencsik T, Papp N. Phytochemical overview and medicinal importance of Coffea species from the past until now. Asian Pacific Journal of Tropical Medicine. 2016;9(12):1127-35. https//doi.org/10.1016/j.apjtm.2016.11.008
- Yeager SE, Batali ME, Guinard JX, Ristenpart WD. Acids in coffee: A review of sensory measurements and meta-analysis of chemical composition. Crit Rev Food Sci Nutr. 2023;63(8):1010-36. https://doi.org/10.1080/10408398.2021.1 957767
- Chen XM, Ma Z, Kitts DD. Effects of processing method and age of leaves on phytochemical profiles and bioactivity of coffee leaves. Food Chem. 2018;249:143-53. https://doi.org/10.1016/j.foodchem.2017.12.073
- Dado AT, Asresahegn YA, Goroya KG. Comparative study of caffeine content in beans and leaves of Coffea arabica using UV/vis spectrophotometer. International Journal of Physical Science. 2019;14(14):171-6. https://doi.org/10.5897/ ijPS2019.4814
- Majedi S, Faraj TA, Ahmed HJ, Hussain FH. A review of biochemical structures of Urtica dioica metabolites and their pharmaceutical effects. Chemical Review and Letters. 2021;4(4):206-12. https://doi.org/10.22034/ crl.2021.316199.1131
- Ibrahim M, Rehman K, Razzaq A, Hussain I, Farooq T, Hussain A, Akash MSH. Investigation of phytochemical constituents and their pharmacological properties isolated from Urtica Genus: Critical review and analysis. Critical Review Eukaryote Gene Expression. 2018;28(1):25-66. https://doi.org/10.1615/CritRevEucaryotGeneExp.2018020389
- Gosudarstvennaya farmakopeya Rossiyskoy Federatsii XIV izdaniya. Tom I [State Pharmacopeia XIVth edition of the Russian Federation. Volume I]. 1814 p. Available from http://www.femb. ru/femb/pharmacopea.php
- GOST 33044-2014 «Printsipy nadlezhashchey laboratornoy praktiki» [Principles of good laboratory practice]. Available from https://docs.cntd.ru/ document/1200115791 (Accessed 17.07.2021)
- Born GV. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962;194:927-9.
- Galiakhmetova EKh, Bashirova LI, Khasanova SR, Kudashkina NV, Nizamova AA, Zhalalova NK, i dr. Vliyanie nekotorykh vidov lekarstvennogo rastitel'nogo syr'ya na sistemu gemostaza in vitro [The effect of certain species of medicinal plant raw material on hemostasis indicators in vitro]. Traditsionnaya meditsina. 2021;1:38-42. https://doi.org/10.54296/18186173_2021_1_38
- Ghalandari S, Kariman N, Sheikhan Z, Mojab F, Mirzaei M, Shahrahmani H. Effect of hydroalcoholic extract of Capsella bursa-pastoris on early postpartum hemorrhage: A clinical trial study. J Altern Complement Med. 2017;23(10):794- 9. https://doi.org/ 10.1089/acm.2017.0095
- Mortel LEH, Austria YVD, Evangelista DJB, Falseco NF, Marasigan VM, Villamin JM, et al. Antithrombotic effect of purified caffeine and ethanol extracts of Coffea Liberica Hiern. leaves in Swiss Albino mice. Asia Pacific Journal of Multidisciplinary Research. 2014;8:35-60.
Authors' information:
Abdullina Guzel Maratovna
Candidate of Biological Sciences, Associate Professor, Associate Professor of the Department of Biochemistry, Bashkir State Medical University
ORCID ID: 0000-0003-4640-3879
E-mail: gmabdullina@mail.ru
Kudashkina Natalia Vladimirovna
Doctor of Pharmaceutical Sciences, Full Professor, Head of the Department of Pharmacognosy with a Course of Botany and Basics of Phytotherapy, Bashkir State Medical University
Scopus ID: 57207299733
ORCID ID: 0000-0002-0280-143X
SPIN: 4256-5502
Author ID: 637292
E-mail: phytoart@mail.ru
Khasanova Svetlana Rashitovna
Doctor of Pharmaceutical Sciences, Professor of the Department of Pharmacognosy with a Course of Botany and Basics of Phytotherapy, Bashkir State Medical University Researcher ID: DWR-9689-2022
Scopus ID: 57212514163
ORCID ID: 0000-0001-7000-8014
SPIN: 7027-0676
Author ID: 465615
E-mail: svet-khasanova@yandex.ru
Ganyev Ruslan Zhakshibaevich
Lecturer of the Department of Organization of Pharmaceutical Business and Pharmacognosy, Osh State University
ORCID ID: 009-0001-6868-4838
SPIN: 3050-1975
E-mail: ganyevr0@gmail.com
Samorodov Aleksandr Vladimirovich
Doctor of Medical Sciences, Full Professor, Head of the Department of Pharmacology with a Course of Clinical Pharmacology, Bashkir State Medical University
ORCID ID: 0000-0001-9302-499X
E-mail: avsamorodov@gmail.com
Chiduku Nikolas
Student of Faculty of Pharmacy, Bashkir State Medical University
Researcher ID: JJE-2281-2023
ORCID ID: 0009-0007-4257-1302
E-mail: nicholaschiduku@gmail.com
Sadykova Farida Valievna
Candidate of Biological Sciences, Associate Professor, Head of the Educational and Experimental Farm of Ufa Forestry Technical College; Associate Professor of the Department of Physiology and General Biology of the Institute of Nature and Human, Ufa University of Science and Technology
ORCID ID: 0000-0002-4749-404х
E-mail: faridalimon@yandex.ru
Information about support in the form of grants, equipment, medications
The authors did not receive financial support from manufacturers of medicines and medical equipment
Conflicts of interest: No conflict
Address for correspondence:
Abdullina Guzel Maratovna
Candidate of Biological Sciences, Associate Professor, Associate Professor of the Department of Biochemistry, Bashkir State Medical University
450103, Russian Federation, Ufa, Lenina Ave., 3
Tel.: +7 (906) 3713902
E-mail: gmabdullina@mail.ru
Materials on the topic:
- ANALYSIS OF ANTIBACTERIAL AND FUNGICIDAL ACTIVITY OF ALLIUM OSHANINII AND ALLIUM SUWOROWII DEPENDING ON THE ALTITUDE OF PLANT GROWTH
- STUDY OF ANTIBACTERIAL ACTIVITY OF EXTRACTS OF DIFFERENT SPECIES OF THE GENUS Allium GROWING IN TAJIKISTAN
- ANTIBACTERIAL, ANTIFUNGAL, ANTIOXIDANT ACTIVITY AND POLYPHENOL CONTENT OF AERIAL PARTS AND BULBS OF ALLIUM SCHUGNANICUM
- DEVELOPMENT OF THE COMPOSITION AND TECHNOLOGY OF COATED TABLETS WITH RHEUM CORDATUM LOSINSK. EXTRACT
- COMPARATIVE CHARACTERISTICS OF ANTIBACTERIAL ACTIVITY OF PLANTS GROWING IN THE CENTRAL PART OF THE REPUBLIC OF TAJIKISTAN
- ANTI-TUMOR ACTIVITY OF DRY EXTRACT PADUS GRAYANAE MAXIM IN VITRO
- ABOUT SIMPLE ANTI-DIABETIC REMEDIES BY AVICENNA
- ANTIOXIDANT ACTIVITY AND CONTENT OF POLYPHENOLS IN ARTEMISIA SCOPARIA WALDST. ET KIT., GROWING UNDER THE CONDITIONS OF WESTERN PAMIR
- MOLECULAR APPROACH TO DETERMINE THE HOT AND COLD TEMPERAMENTS IN PLANTS ACCORDING AVICENNA CONCEPTS, THE ROLE OF MAGNESIUM