Pediatrics

doi: 10.25005/2074-0581-2018-20-1-63-68
MANIFESTATION OF POLYMORPHISM OF LIPID METABOLISM GENES TO IDENTIFY THEIR ROLE IN THE DEVELOPMENT OF VASCULAR DISEASES IN CHILDREN BORN OF MOTHERS WITH METABOLIC SYNDROME

V.V. Nikitina, О.P. Zhukhareva, I.L. Ivanenko, G.P. Gladilin, E.V. Volodko

Department of Clinical Laboratory Diagnostics, Saratov State Medical University named after V. I. Razumovsky, Saratov, Russian Federation

Objective: To study polymorphism of lipid metabolism genes as a predictor of the development of vascular disorders in newborns from the mothers with metabolic syndrome.

Methods: 48 people were surveyed: 24 mothers and their newborn's children (24 children), of whom 18 (9 mothers and 9 children) went to the control group. A biochemical blood test with the study of carbohydrate, lipid and protein metabolism on the automatic biochemical analyzer Olympus AU 680 was conducted in all the blood serum. The material for genetic research was venous blood. Genetic polymorphisms determined using the reagent kit “Lipid exchange. Additional profile” with the definition of polymorphisms АВСА1, АРОС3, LPL, PON1 by pyrosequencing method.

Results: According to the research in the blood serum of children in the violations of protein and carbohydrate metabolism is not revealed. The increase in bilirubin is explained by the physiological jaundice of the newborns, which show extracting of children from the maternity hospital. Women in childbirth and their newborn children, despite the permissible values of the studied indices of lipid metabolism, a high level of atherogenicity coefficient was observed. In the conduct of molecular genetic research “Lipid exchange. Additional profile” by the method of pyrosequencing women in childbirth and their newborn children in groups 2 and 3 it turned out that the examined children of the protective allele in polymorphism rs2230806 (variant GG) was not identified, that is associated with the risk of cardiovascular diseases, while mothers were present. Women in childbirth and their newborn babies were indentified allele risk in polymorphism rs5128, a homozygote that is associated with the development of the metabolic syndrome. A protective allele in polymorphism rs328 was not found, which indicates the possible development of coronary heart disease, ischemic and athero-thrombotic stroke. In children, a risk allele in polymorphism rs662 was identified in the heterozygous state – a marker of increased risk of cardiovascular and atherosclerotic diseases, which was absent in mothers.

Conclusions: In the case of newborn children, the alleles of risk or the absence of protective alleles of the above-mentioned polymorphisms, should be classified as a risk group if possible development of cardiovascular pathology.

Keywords: Metabolic syndrome, genetic polymorphisms, newborn children, cardiovascular pathology.

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Authors informations:

Nikitina Victoria Viktorovna, Candidate of Medical Sciences, Associate Professor of the Department of Clinical Laboratory Diagnostics, Saratov State Medical University named after V.I. Razumovsky

Zhukhareva Olga Pavlovna, Postgraduate Student of the Department of Clinical Laboratory Diagnostics, Saratov State Medical University named after V.I. Razumovsky

Ivanenko Irina Lvovna, Candidate of Medical Sciences, Associate Professor of the Department of Clinical Laboratory Diagnostics, Saratov State Medical University named after V.I. Razumovsky

Gladilin Gennady Pavlovich, Doctor of Medical Sciences, Full Professor, Head of the Department of Clinical Laboratory Diagnostics, Saratov State Medical University named after V.I. Razumovsky

Volodko Elizaveta Vladimirovna, Attending Physician of the Department of Clinical Laboratory Diagnostics, Saratov State Medical University named after V.I. Razumovsky

Address for correspondence:

Nikitina Victoria Viktorovna

Candidate of Medical Sciences, Associate Professor of the Department of Clinical Laboratory Diagnostics, Saratov State Medical University named after V.I. Razumovsky

410012, Russian Federation, Saratov, str. Moscowskaya, building 134/146, Apt. 199

Tel.: (+7) 987 3350746

E-mail: vik-nik72@mail.ru

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