Effects of amplifier liver regeneration on the currency in vivo reperfusion injury

E. Iskenderov, A. Kandoga, K. Mende

Research Centre of Surgery named after academician. M.A.Topchibashev, Baku, Azerbaijan
University Hospital Grosshadern, Munich, Germany

Microcirculation disturbance is the main target in liver lesion in reperfusion injury. Authors studied the efficacy of ALR on sinusoidal perfusion.

The studies were conducted in mice type C57BL / 6 mice weighing 18-25 grams, in experimental conditions. Experimental animals divided into 3 groups of 6 animals each. In the first (main) group a model lobar warm ischemia is created. After 90 minutes of ischemia, 100 μg ALR was administered to animal, followed by a reperfusion. In the second group instead ALR warm saline was administered, and the third group only laparotomy was made. The main group of microcirculatory disorders were much easier, and intensity of blood flow was markedly different from that in the liver of mice of control group

In calculation of sinusoidal blood flow intensity, it was found that the percentage of non-perfusional sinusoids is 22 ± 4% and was significantly different from the rate in the group where preliminary treatment was not given. On blood chemistry AST and ALT activity was respectively 50% and 28% lower compared with the control group.

The results showed a beneficial effect on recovery ALR impaired hepatic blood flow and the functional activity of liver after ischemia and reperfusion.

Keywords: liver, transplantation, reperfusion injury, microcirculation, sinusoidal perfusion.

Download file:

---